matrigel-coated transwell chambers diameter pore size  (Corning Life Sciences)

Journal: Materials Today Bio

Article Title: A MnO 2 -based tumor-seeking nanoplatform for enhanced chemoimmunotherapy against 4T1 breast cancer

doi: 10.1016/j.mtbio.2025.102000

Figure Lengend Snippet: Cellular uptake of Squ@APS NPs and Squ@APS-IR820 NPs and transwell invasion assays. Fluorescence image of 4T1 cells co-cultured with Squ/C6@APS (A) and Squ/C6@APS-IR820 (B) at 1 h, 3 h, and 6 h, respectively. Blue and green represent DAPI and C6 fluorescence, which stand for the position of cell nucleus and NPs. The endocytosis of Squ/C6@APS (C) and Squ/C6@APS-IR820 (D) by 4T1 cells was visualized by flow cytometry. Blank represents cells treated with the same volume of medium, which was used as a control (Red). The mean fluorescence intensity (MFI) value (E) of 4T1 cells co-cultured with Squ/C6@APS and Squ/C6@APS-IR820NPs for 1 h, 3 h, and 6 h. Fluorescence image (F) and the mean fluorescence intensity (MFI) value (G) of 4T1 cells co-cultured with Squ/C6@APS-IR820 NPs for 3 h pretreated with 100 μM of probenecid or 50 μM of doxorubicin for 30 min. Invasion of 4T1 cells treated with saline (H), Squ@APS-IR820 NPs (I), MnO 2 @APS-IR820 (J), and Squ@APS-IR820 NPs + MnO 2 @APS-IR820 (K). Invasive cells in every field (L). Data are presented as means ± standard deviation (SD). Differences among groups were evaluated by one-way ANOVA analysis. ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05. ns, no significant difference. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The invasive capacity of 4T1 cells was assessed using matrigel-coated transwell chambers (6.5 mm diameter, 8 μm pore size; Corning).

Techniques: Fluorescence, Cell Culture, Flow Cytometry, Control, Saline, Standard Deviation

Journal: Cancer Cell International

Article Title: A LINC00472-encoded polypeptide impedes migration and proliferation through modulation of the HDAC2/SP1 axis in non-small cell lung cancer cells

doi: 10.1186/s12935-025-03901-z

Figure Lengend Snippet: LINC00472-encoded polypeptide suppresses NSCLC proliferation, migration, and invasion. ( A - F ) H1299 and A549 NSCLC cells were transfected with vector (Vec), wild-type LINC00472-ORF (LINC00472-ORFwt), or translation-deficient mutant LINC00472-ORF (LINC00472-ORFmut). ( A ) Cell viability assessment by CCK8 assay at 48 h post-transfection. ( B ) DNA synthesis quantification via EdU incorporation assay at 48 h post-transfection. EdU images: 100× magnification. ( C ) IF analysis of MMP9 expression levels at 48 h post-transfection. IF image, 200× magnification. ( D ) Migration capacity evaluation using wound-healing assay with imaging at 0 h and 24 h post-scratch. ( E ) Cell migration potential measurement by transwell assay at 24 h post-seeding. Transwell images: 100× magnification. ( F ) Invasion capacity analysis using Matrigel-coated transwell assay at 36 h post-seeding. Transwell images: 100× magnification. *** indicates a P -value of < 0.001 when compared to the Vec group, while ns denotes no significant difference compared to the Vec group

Article Snippet: Transfected H1299 and A549 cells were resuspended in serum-free medium and seeded into 8-μm pore transwell chambers (Corning), with complete medium containing 10% FBS added to the lower chamber.

Techniques: Migration, Transfection, Plasmid Preparation, Mutagenesis, CCK-8 Assay, DNA Synthesis, Expressing, Wound Healing Assay, Imaging, Transwell Assay

Journal: Cancer Cell International

Article Title: A LINC00472-encoded polypeptide impedes migration and proliferation through modulation of the HDAC2/SP1 axis in non-small cell lung cancer cells

doi: 10.1186/s12935-025-03901-z

Figure Lengend Snippet: Regulatory role of LINC00472-ORF polypeptide in NSCLC cells through SP1 modulation. ( A - D ) Analysis of PCNA expression via immunofluorescence (IF) assay ( A ), assessment of cell migration capacity ( B ), and evaluation of MMP2 and MMP9 levels through IF microscopy ( C and D ) in H1299 and A549 NSCLC cells post-introduction of vector + CON, LINC00472-ORF + CON, or LINC00472-ORF + SP1 constructs. IF image, 200× magnification. Transwell images: 100× magnification. ( E - H ) H1299 and A549 cells were treated with Mithramycin A, either alone or in combination with LINC00472-ORF. The PCNA, MMP2, and MMP9 expressions were analyzed using IF assays, while cell migratory ability was assessed through a transwell assay. IF image, 200× magnification. Transwell images: 100× magnification. In panel A-D, * / ** / *** indicates a P -value of < 0.05/0.01/0.001 when compared to the Vector + CON group, while # / ## / ### indicates a P -value of < 0.05/0.01/0.001 when compared to the LINC00472-ORF + CON group. In panel ( E - H ), *** indicates a P -value of < 0.001 when compared to the Vector + DMSO group, while ns denotes no significant difference compared to the Vector + Mithramycin A group

Article Snippet: Transfected H1299 and A549 cells were resuspended in serum-free medium and seeded into 8-μm pore transwell chambers (Corning), with complete medium containing 10% FBS added to the lower chamber.

Techniques: Expressing, Immunofluorescence, Migration, Microscopy, Plasmid Preparation, Construct, Transwell Assay